Unearthing the ecology of soil microorganisms using a high resolution DNA-SIP approach to explore cellulose and xylose metabolism in soil

Unearthing the ecology of soil microorganisms using a high resolution DNA-SIP approach to explore cellulose and xylose metabolism in soil

Blog Article

We explored microbial contributions to decomposition using a sophisticated approach to DNA Stable Isotope Probing (SIP).Our experiment evaluated the dynamics and ecological characteristics of functionally veuve ambal rose defined microbial groups that metabolize labile and structural C in soils.We added to soil a complex amendment representing plant derived organic matter substituted with either 13C-xylose or 13C-cellulose to represent labile and structural C pools derived from abundant components of plant biomass.We found evidence for 13C-incorporation into DNA from 13C-xylose and 13C-cellulose in 49 and 63 operational taxonomic units (OTUs), respectively.

The types of microorganisms that assimilated 13C in the 13C-xylose treatment changed rosy teacup dogwood over time being predominantly Firmicutes at day 1 followed by Bacteroidetes at day 3 and then Actinobacteria at day 7.These 13C-labeling dynamics suggest labile C traveled through different trophic levels.In contrast, microorganisms generally metabolized cellulose-C after 14 days and did not change to the same extent in phylogenetic composition over time.Microorganisms that metabolized cellulose-C belonged to poorly characterized but cosmopolitan soil lineages including Verrucomicrobia, Chloroflexi and Planctomycetes.